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NASC ID: N849254

Name: WiscDsLox233237_03K

ABRC stock number: CS849254

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox233237_03K.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G78390.1 (at chr 1 pos 29492945 (C/29492945-29493003) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 83-100 bps onto the T-DNA. Pools: P 0, R 2, C 5 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453581.

Wiscseq_DsLox233237_03K.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT2G26180.1 (chr 2 pos 11145066 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG795911.

Wiscseq_DsLox233237_03K.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G27315.1 and AT2G27320.1-P355 (chr 2 pos 11691049 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG795912.

Wiscseq_DsLox233237_03K.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G18810.1 (at chr 3 pos 6481679 (C/6481679-6481747) on the TAIR10). The sequences' 1-69 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 0, R 2, C 5 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452383.

Wiscseq_DsLox233237_03K.4 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G22630.1-P355 and AT3G22640.1-UTR3 (chr 3 pos 8011317 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG795913.

Donation Date: 2006-09-04

Donated by:  University of Wisconsin, Madison Patrick Krysan
University of Wisconsin, Madison Richard Amasino
University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox233237_03K

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • WiscDsLox233237_03K
  • WiscDsLox233237_03K